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Image Search Results
Journal: Frontiers in Microbiology
Article Title: An Endogenous Retroviral LTR-Derived Long Noncoding RNA lnc-LTR5B Interacts With BiP to Modulate ALV-J Replication in Chicken Cells
doi: 10.3389/fmicb.2021.788317
Figure Lengend Snippet: Characterization of the lnc-LTR5B. (A) Schematic map of lnc-LTR5B. lnc-LTR5B is located on chromosome 17 and is flanked by the coding genes TUBB4B and ENTPD2L. (B) Dual-luciferase assays of lnc-LTR5B promoter activity in HEK293T cells transfected with the indicated plasmids. Schematic illustration of truncation constructs of lnc-LTR5B promoter region (−2000 to +198 relative to TSS). Data are presented as the mean±SD, n =3; ** p <0.01, *** p <0.001 (two-tailed unpaired Student’s t test). (C) Confocal RNA FISH images showing the distribution of lnc-LTR5B in DF-1 cells. The lnc-LTR5B probes labeled with CY3 (red), and nuclei are stained with 4', 6-diamidino-2-phenylindole (DAPI; blue), Scale bars, 10μm.
Article Snippet: The lnc-LTR5B probes labeled with
Techniques: Luciferase, Activity Assay, Transfection, Construct, Two Tailed Test, Labeling, Staining